​Scaling down single-cell reactions to the nanoliter level is critical to minimize the risk of contamination, increase reaction efficiency, and reduce costs.Researchers from the Single-cell Center of the Qingdao Institute of Bioenergy and Bioprocess Technology of the Chinese Academy of Sciences have developed a centrifugally driven system for precise manipulation of nanoliter liquids in single...

With the rapid development of genome synthesis and editing technologies, droplet-based single-cell phenotype screening has become increasingly important. However, precise and high-throughput distribution of target droplets into specific macroscopic media for downstream multi-omics analysis remains a technical challenge.Researchers from the Qingdao Institute of Bioenergy and Bioprocess Technolog...

​In response to the escalating challenges posed by the high drug resistance of rapidly growing mycobacteria (RGM), a collaborative team from the Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT) of the Chinese Academy of Sciences, the Beijing Chest Hospital, and the Qingdao Single-Cell Biotech has achieved a breakthrough in rapid antimicrobial susceptibility testing (AST) of RG...

​Cyanobacteria, as unique photosynthetic prokaryotic organisms, possess the extraordinary ability to directly convert sunlight, seawater, and carbon dioxide into a wide variety of bio-based products.Currently, the evaluation of cyanobacterial growth rate is mainly based on the time-consuming and labor-intensive monitoring of cell number or biomass accumulation after strain isolation and cultiv...

The presence of viable bacteria in the blood, i.e., bacteremia, when not controlled properly can lead to bloodstream infection (BSI) and sepsis, a syndromic inflammatory response.Rapid and accurate antimicrobial prescriptions are critical to decrease mortality significantly for BSI patients. However,the traditional antimicrobial susceptibility testing (AST) for BSI is time-consuming and tedious...

Static droplet array (SDA) is a necessary and integral tool when it comes to high-capacity screening tests, or assays, for identifying and targeting specific cells or analytes (substances whose chemical composition is the target of identification). However, extraction and collection of the target droplets that contain unique analytes or cells from the SDA remains one major technical bottleneck ...

Sequencing the genome of single bacterial cells has long been technically difficult due to the seemingly incorrigible bias in the gene amplification stage of the process, making it hard to produce high-coverage genome sequence from precisely just one bacterial cell.A new process developed by researchers at the Single-Cell Center, Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT)...

​Probiotics claim to host a slew of health benefits for a person. Rapid expansion of the probiotics industry demands fast, sensitive, comprehensive and low-cost strategies for quality assessment.A research team led by the Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT) of the Chinese Academy of Sciences (CAS) has developed a new approach called Single-Cell Identification, Via...

​Rapid-results testing has become an increasingly important part of our fast-paced world. Utilizing droplet microfluidics allows for quicker point-of-care testing (POCT) but is not always as accurate, and currently requires an amount of external effort for proper handling.The Integrated Portable Droplets system, or iPODs, developed by researchers from the Single-Cell Center at Qingdao Institut...

Effective, accurate and quick ways to screen and sort microbes are in short supply. Most methods available now rely on additional labeling steps to sort bacteria, which are typically time-consuming and cannot work well for industrial-scale breeding.The need for accuracy and speed are met using a label-free droplet-based integrated microfluidic platform that screens bacterial growth based on phe...