​Raman spectroscopy is a scattering technique that relies on the inelastic scattering of photons. The excitation of molecular bonds results in the shifts of the energy of laser photons and thus frequency change of the incident light. Therefore, a compound can have a specific Raman spectrum, while a Single-cell Raman Spectrum (SCRS) can depict the overall profile of metabolites in the cell, i.e...

Researchers from the Single-Cell Center at the Qingdao Institute of Bioenergy and Bioprocess Technology of the Chinese Academy of Sciences, together with collaborators, developed an artificial intelligence-assisted Raman-activated cell sorting (AI-RACS) system. This innovative system automated the isolation and functional analysis of aluminum-tolerant microorganisms (ATMs) from acidic soil, mar...

​In the competitive landscape of the yeast fermentation industry, rapid and accurate quality assessment is crucial for optimizing production and enhancing commercial competitiveness.Researchers from Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences (CAS) and Tianjin Agricultural College have introduced an innovative approach utilizing single-cell Ra...

​Scaling down single-cell reactions to the nanoliter level is critical to minimize the risk of contamination, increase reaction efficiency, and reduce costs.Researchers from the Single-cell Center of the Qingdao Institute of Bioenergy and Bioprocess Technology of the Chinese Academy of Sciences have developed a centrifugally driven system for precise manipulation of nanoliter liquids in single...

With the rapid development of genome synthesis and editing technologies, droplet-based single-cell phenotype screening has become increasingly important. However, precise and high-throughput distribution of target droplets into specific macroscopic media for downstream multi-omics analysis remains a technical challenge.Researchers from the Qingdao Institute of Bioenergy and Bioprocess Technolog...

​Cyanobacteria, as unique photosynthetic prokaryotic organisms, possess the extraordinary ability to directly convert sunlight, seawater, and carbon dioxide into a wide variety of bio-based products.Currently, the evaluation of cyanobacterial growth rate is mainly based on the time-consuming and labor-intensive monitoring of cell number or biomass accumulation after strain isolation and cultiv...

​Microalgae—which are major contributors to global photosynthesis and primary productivity—serve as promising chassis cells in synthetic biology.In a study published in Plant Communications, researchersfrom the Qingdao Institute of Bioenergy and Bioprocess Technology of the Chinese Academy of Sciences have unraveled the distribution pattern and dynamic changes in DNA N6-methyladenine (6mA) a...

​In response to the escalating challenges posed by the high drug resistance of rapidly growing mycobacteria (RGM), a collaborative team from the Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT) of the Chinese Academy of Sciences, the Beijing Chest Hospital, and the Qingdao Single-Cell Biotech has achieved a breakthrough in rapid antimicrobial susceptibility testing (AST) of RG...

The presence of viable bacteria in the blood, i.e., bacteremia, when not controlled properly can lead to bloodstream infection (BSI) and sepsis, a syndromic inflammatory response.Rapid and accurate antimicrobial prescriptions are critical to decrease mortality significantly for BSI patients. However,the traditional antimicrobial susceptibility testing (AST) for BSI is time-consuming and tedious...

Static droplet array (SDA) is a necessary and integral tool when it comes to high-capacity screening tests, or assays, for identifying and targeting specific cells or analytes (substances whose chemical composition is the target of identification). However, extraction and collection of the target droplets that contain unique analytes or cells from the SDA remains one major technical bottleneck ...